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Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
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Bioengineering
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JoVE Journal Bioengineering
Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

DOI:
10.3791/55030-v

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09:28 min

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January 10, 2017

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, , , , ,
1Department of Chemical and Biomolecular Engineering,University of California Berkeley, 2California Institute for Quantitative Biosciences (QB3),University of California Berkeley

Chapters

  • 00:05Title
  • 00:43Preparation of Nucleic Acid Suspensions of Single Walled Carbon Nanotubes (SWNTs)
  • 02:34Amphiphilic Polymer Suspensions of SWNTs
  • 03:54Prepare BSA-biotin Coated Microscope Slides
  • 06:10Fluorescence Spectroscopy and Microscopy of SWNTs
  • 08:06Results: Fluorescence Response of Nanosensors
  • 08:48Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol for engineering the corona phase of near infrared fluorescent single walled carbon nanotubes (SWNTs) using amphiphilic polymers and DNA to develop sensors for molecular targets without known recognition elements.

Tags

Keywords: Single Walled Carbon NanotubesBio-mimetic PolymersNear-infrared FluorescenceMolecular SensingDopamine DetectionNucleic Acid SuspensionUltrasonicationCentrifugationAmphiphilic Polymer SuspensionsSodium Cholate

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