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JoVE Journal Biology
Cryosectioning af sammenhængende regioner en enkelt mus Skeletal Muscle for genekspression og histologiske analyser
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Cryosectioning af sammenhængende regioner en enkelt mus Skeletal Muscle for genekspression og histologiske analyser

Article DOI: 10.3791/55058
December 12th, 2016 •
1
1Department of Pharmaceutical and Biomedical Sciences, University of Georgia

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December 12th, 2016

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Konsekutive cryo-sektioner er samlet for at muliggøre histologiske applikationer og berigelse af RNA til genekspression målinger med tilstødende regioner fra en enkelt mus skeletmuskel. Høj kvalitet RNA fås fra 20 - 30 mg puljede kryosnit og målinger direkte sammenlignes på tværs af applikationer.

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