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Journal / Immunology and Infection / Dextran verbetert de efficiëntie van de…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Dextran verbetert de efficiëntie van de lentivirale transductie van lymfkliertest en menselijke primaire NK-cellen
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Dextran verbetert de efficiëntie van de lentivirale transductie van lymfkliertest en menselijke primaire NK-cellen

Article DOI: 10.3791/55063-v • 08:42 min • January 15th, 2018
January 15th, 2018 •
1, 2, 3, 4, 5, 6, 6, 1,5,6,7
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute, The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute, The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute, The Blood Center of Wisconsin, 5Department of Microbiology and Immunology, The Medical College of Wisconsin, 6Department of Pediatrics, The Medical College of Wisconsin, 7Department of Medicine, The Medical College of Wisconsin

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Het doel van deze studie was om formuleren van technologieën die het mogelijk voor succesvolle gene signaaltransductie in primaire natural killer (NK) cellen maken. De dextran-gemedieerde lentivirale transductie van mens of muis primaire NK cellen leidt tot hogere rendementen van gen expressie. Deze methode van gene transductie zal sterk verbeteren NK cel genetische manipulatie.

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Immunologie kwestie 131 signaaltransductie primaire NK-cellen dextran lentivirus gemodificeerde immunotherapie
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