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Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction
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Neuroscience
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JoVE Journal Neuroscience
Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction

Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction

DOI:
10.3791/55122-v

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17:05 min

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July 08, 2017

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, , , ,
1Laboratory of Biophysics of Synaptic Processes, Kazan Scientific Centre, Kazan Institute of Biochemistry and Biophysics,Russian Academy of Sciences, 2Open Laboratory of Neuropharmacology,Kazan Federal University, 3Department of Radiophotonics and Microwave Technologies,A.N. Tupolev Kazan National Research Technical University, 4Department of Medical and Biological Physics,Kazan State Medical University

Chapters

  • 00:00Title
  • 00:06Introduction
  • 01:03Preparing of a Dye Loading Solution
  • 01:53Dye Loading Procedure
  • 05:40Praraing the Tissue for Microscopy
  • 08:22Video Capture with Digital CCD Camera
  • 10:06Data Analysis
  • 14:13Results
  • 14:56Discussion

Summary

Automatic Translation

Automatic Translation

Here, we describe a method for loading a calcium-sensitive dye through the frog nerve stump into the nerve endings. We also present a protocol for the recording and analysis of fast calcium transients in the peripheral nerve endings.

Tags

Keywords: Calcium DyeFrog Nerve EndingsNeuromuscular JunctionOptical RecordingCalcium TransientCalcium SignalingHEPESOregon Green BAPTARinger’s SolutionNerve StumpNerve EndingsMuscle Preparation

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