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In Vitro Differentiation of Mature Myofibers for Live Imaging
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Differentiation of Mature Myofibers for Live Imaging

In Vitro Differentiation of Mature Myofibers for Live Imaging

DOI:
10.3791/55141-v

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08:12 min

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January 07, 2017

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, , ,
1Instituto de Medicina Molecular, Faculdade de Medicina,Universidade de Lisboa, 2Myology Research Center, UM76-INSERM U974-CNRS FRE 361,Sorbonne University, UPMC University of Paris 6

Chapters

  • 00:05Title
  • 00:46Neonatal Mouse Hind-limb Dissection
  • 02:09Myoblast Isolation
  • 04:09Myofiber Differentiation and Immunostaining
  • 06:10Results: Developmental Stages of the Myoblast Culture
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

Muscle cells are among the most complex eukaryotic cells. We present a protocol for the in vitro differentiation of highly mature myofibers that allows for genetic manipulation and clear imaging during all developmental stages.

Tags

Keywords: In Vitro DifferentiationMature MyofibersLive ImagingNeonatal Mouse MyoblastsTransverse Shear TriadSarcomere AssemblyMyofibril AssemblyOrganelle PositioningTime-lapse MicroscopyEnzymatic DigestionCell IsolationFibroblast AdhesionBasement Membrane Coating

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