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Journal / Immunology and Infection / Legionella pneumophila membrane externe…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Legionella pneumophila membrane externe Vésicules
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Legionella pneumophila membrane externe Vésicules: Isolement et analyse de leur potentiel pro-inflammatoire sur Macrophages

Article DOI: 10.3791/55146-v • 08:35 min • February 22nd, 2017
February 22nd, 2017 •
1, 1, 1, 1, 1, 1,2,3
1Institute for Lung Research, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, 2German Center for Lung Research, 3Department of Medicine, Pulmonary and Critical Care Medicine, University Medical Center Giessen and Marburg

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Ici, nous décrivons la purification de Legionella pneumophila (L. pneumophila) des vésicules de membrane externe (OMVS) à partir de cultures liquides. Ces vésicules purifiées sont ensuite utilisés pour le traitement des macrophages pour analyser leur potentiel pro-inflammatoire.

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Infection numéro 120, Des vésicules de membrane externe macrophage la purification l'ultracentrifugation différentielle la réponse pro-inflammatoire la replication bactérienne
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