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January 24, 2017
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Summary
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Mitokondri onları sitozolik Ca şekillendirmek için 2+ hücre içinde sinyal sağlayan (2+ Ca) kalsiyum tecrit kendi iç zarı (Δ Ψm) arasında elektrokimyasal potansiyeli kullanabilir. Floresan boyalar ve konfokal mikroskopi kullanılarak canlı hücreler eş zamanlı olarak ölçüm mitokondri Ca 2 + alımı ve ΔΨ m için bir yöntem tarif eder.
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Cite this Article
McKenzie, M., Lim, S. C., Duchen, M. R. Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy. J. Vis. Exp. (119), e55166, doi:10.3791/55166 (2017).
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