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JoVE Journal Biology
Floresan Mikroskopi Canlı hücreler Potansiyel Mitokondriyal Kalsiyum ve Mitokondriyal Membran Eşzamanlı Ölçümü
Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
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Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
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Floresan Mikroskopi Canlı hücreler Potansiyel Mitokondriyal Kalsiyum ve Mitokondriyal Membran Eşzamanlı Ölçümü

Article DOI: 10.3791/55166
January 24th, 2017 •
1,2, 1, 3
1Centre for Genetic Diseases, Hudson Institute of Medical Research, 2The Department of Molecular and Translational Sciences, Monash University, 3Department of Physiology, University College London

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January 24th, 2017

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Mitokondri onları sitozolik Ca şekillendirmek için 2+ hücre içinde sinyal sağlayan (2+ Ca) kalsiyum tecrit kendi iç zarı (Δ Ψm) arasında elektrokimyasal potansiyeli kullanabilir. Floresan boyalar ve konfokal mikroskopi kullanılarak canlı hücreler eş zamanlı olarak ölçüm mitokondri Ca 2 + alımı ve ΔΨ m için bir yöntem tarif eder.

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