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Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

DOI:
10.3791/55213-v

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10:39 min

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April 22, 2017

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, , , ,
1Laboratory of Genetics,University of Wisconsin-Madison, 2Department of Biological Sciences,Tata Institute of Fundamental Research, 3Department of Genetics, Cell Biology, and Development,University of Minnesota

Chapters

  • 00:05Title
  • 00:50Pre-selection of Females and Preparation of Maturation Medium
  • 02:29Dissection of Oocytes and Initiation of In Vitro Culture
  • 04:56Oocyte Microinjection
  • 05:52Oocyte Maturation and Defolliculation
  • 07:18Fertilization of In Vitro-cultured Oocytes
  • 08:15Results: Maternal Gene Product Manipulation Using In Vitro Oocyte Maturation
  • 09:36Conclusion

Summary

Automatic Translation

Automatic Translation

An optimized protocol for the in vitro maturation of zebrafish oocytes used for the manipulation of maternal gene products is presented here.

Tags

Keywords: In Vitro Oocyte MaturationZebrafishMaternal Gene ProductsDevelopmental BiologyFertilizationViable EmbryosMating TankLeibovitz’s L-15 MediumDHPGentamicinTricaineOocyte DissectionLight Cycle

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