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Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
JoVE Journal
Genetics
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JoVE Journal Genetics
Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

16,437 Views

10:50 min

February 25, 2017

DOI:

10:50 min
February 25, 2017

16401 Views
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Summary

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We describe a method to sort single mammalian cells and to quantify the expression of up to 96 target genes of interest in each cell. This method includes the use of internal qPCR standards to enable the estimation of absolute transcript counts.

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