Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal

A subscription to JoVE is required to view this content.

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Article DOI: 10.3791/55229 11:26 min May 22nd, 2017
May 22nd, 2017



Here, we present a combinatorial approach for classifying neuronal cell types prior to isolation and for the subsequent characterization of single-cell transcriptomes. This protocol optimizes the preparation of samples for successful RNA Sequencing (RNA-Seq) and describes a methodology designed specifically for the enhanced understanding of cellular diversity.



Single-cell RNA-Seq Defined Subsets Retinal Ganglion Cells Fluorescently Labeled Single Cells Live Whole Mount Retinas Neuroscience Genetic Markers Retinal Tissue Dissociation Cellular Diversity Cellular Function Health And Disease Patch-clamp RNA Isolation Steps Cornea Sclera Optic Nerve Retina Vitreous
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter