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Neuroscience

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Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
 

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Article DOI: 10.3791/55229
May 22nd, 2017

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Summary May 22nd, 2017

Here, we present a combinatorial approach for classifying neuronal cell types prior to isolation and for the subsequent characterization of single-cell transcriptomes. This protocol optimizes the preparation of samples for successful RNA Sequencing (RNA-Seq) and describes a methodology designed specifically for the enhanced understanding of cellular diversity.

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