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Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry
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Neuroscience
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JoVE Journal Neuroscience
Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

DOI:
10.3791/55254-v

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08:36 min

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March 23, 2017

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, , , ,
1Department of Anatomy and Cell Biology,The University of Western Ontario, 2Department of Chemistry and Biochemistry,The University of Western Ontario

Chapters

  • 00:05Title
  • 00:42Tissue Preparation and Sectioning
  • 02:08Sublimator Apparatus Set-up
  • 04:57Sublimation
  • 06:57Results: Matrix-assisted Laser Desorption/Ionization (MALDI) Imaging Mass Spectrometry (IMS) of Gangliosides in the Rat Brain
  • 07:31Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for the sublimation of DAN matrix onto rat brain tissue for the detection of gangliosides using MALDI Imaging Mass Spectrometry is presented.

Tags

Keywords: GangliosidesMALDI Imaging Mass SpectrometrySublimationDAN MatrixCryostat SectioningLipid DetectionNeurobiology

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