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In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
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JoVE Journal Developmental Biology
In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

DOI:
10.3791/55268-v

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08:21 min

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March 16, 2017

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1Department of Biomedical Sciences, School of Veterinary Medicine,University of Pennsylvania

Chapters

  • 00:05Title
  • 00:49Transitioning hESCs to Feeder-free Conditions
  • 02:59hESC Differentiation Using BMP4/A/P
  • 04:02Trophoblastic Cell Transfection Preparation
  • 04:41Transfection Using siRNAs
  • 06:33Results: Rapid Differentiation for Visualization and Analysis
  • 07:51Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to efficiently generate human trophoblastic cells from human pluripotent stem cells using bone morphogenic protein 4 and inhibitors of the Activin/Nodal pathways. This method is suitable for the efficient differentiation of human pluripotent stem cells and can generate large quantities of cells for genetic manipulation.

Tags

Keywords: Human Pluripotent Stem CellsTrophoblastic CellsBone Morphogenic 4Nodal Signaling PathwaysPlacental FormationExtracellular MatrixMouse Embryonic Fibroblast-conditioned MediumBFGFDispaseDifferentiation Medium

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