Low Oxygen Incubation
Low Oxygen Incubation - Related Videos
09:08
Detecting Reactive Oxygen Species
0 Views •
2023
Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are many deleterious effects associated with unchecked ROS production, including structural damage to DNA and other biological molecules. However, ROS can also be mediators of physiological signaling. There is accumulating evidence that ROS play significant roles in everything from activation of transcription factors to the mediation of...
10:11
The Benthic Exchange of O2, N2 and Dissolved Nutrients Using Small Core Incubations
0 Views •
Cited by 13 •
2016
Here, we present small core incubations for the measurement of sediment-water gas and solute exchange. These will provide reliable measurements of sediment-water exchange that assess the role of sediment in influencing biological and biogeochemical processes in aquatic ecosystems.
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
0 Views •
Cited by 28 •
2011
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
10:30
Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging
0 Views •
Cited by 17 •
2017
Once removed from the body, neuronal tissue is greatly affected by environmental conditions, leading to eventual degradation of the tissue after 6 - 8 h. Using a unique incubation method, which closely monitors and regulates the extracellular environment of the tissue, tissue viability can be significantly extended for >24 h.
02:55
Measuring Neural Activity in a Mouse Brain Slice after Prolonged Incubation
0 Views •
2025
This video demonstrates a technique for measuring neuronal activity in mouse brain slices using calcium imaging and electrophysiological recordings. By perfusing the tissue with potassium ions and recording intracellular calcium changes through high-speed digital imaging, this method reveals real-time neuronal responses.
FAQs
Oxygen availability can change cell differentiation, proliferation, tissue patterning, and embryo development. These effects arise because developmental systems respond to the surrounding oxygen environment, making oxygen a variable that can influence how cells and tissues progress. Comparing cultures maintained under different oxygen levels helps identify oxygen-sensitive signaling pathways and links environmental conditions with developmental outcomes.
Oxygen concentration is only one part of the culture environment. A hypoxia-controlled incubator regulates oxygen together with carbon dioxide, temperature, and humidity so that oxygen availability can be examined under reproducible conditions. Controlling these variables helps researchers attribute differences in development, proliferation, differentiation, or patterning to oxygen conditions rather than to uncontrolled changes in the surrounding culture environment.
Paired comparisons can show whether a developmental response is sensitive to oxygen availability. Differences between the two conditions may expose changes in cell proliferation, differentiation, tissue patterning, or embryo development, while also helping investigators identify oxygen-sensitive signaling pathways. This comparison provides a way to connect altered oxygen environments with normal developmental processes or developmental abnormalities.
Researchers place the selected cells, tissues, or embryos in a hypoxia-controlled incubator and establish the intended oxygen concentration while regulating carbon dioxide, temperature, and humidity. They then maintain the cultures under those conditions and compare them with cultures maintained at atmospheric oxygen. Examining developmental or cellular differences between groups provides the experimental outcome.
This approach is useful when investigators need to examine how oxygen environments affect developing biological systems. It can be applied to cells, tissues, or embryos to study differentiation, proliferation, tissue patterning, and embryo development. The method is especially informative when the goal is to model physiological or developmental oxygen conditions and assess their relationship to normal development or abnormalities.
Observed changes should be considered in relation to the oxygen condition and the matched comparison culture. Altered differentiation, proliferation, tissue patterning, or embryo development may indicate oxygen-sensitive developmental responses, but the controlled incubator conditions provide the basis for interpreting those differences consistently. Such results can help clarify how changing oxygen environments contribute to normal development or developmental abnormalities.