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Journal / Immunology and Infection / System til Effekt og cytotoksicitet Screening af…
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Immunology and Infection

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JoVE Journal Immunology and Infection
System til Effekt og cytotoksicitet Screening af inhibitorer Målretning Intracellulær
Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
Journal (Immunology and Infection)
Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
Journal (Immunology and Infection)
Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
 
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System til Effekt og cytotoksicitet Screening af inhibitorer Målretning Intracellulær

Article DOI: 10.3791/55273-v • 09:57 min • April 5th, 2017
April 5th, 2017 •
1, 1
1Department of Medicine, University of British Columbia

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Vi har udviklet en modulær højkapacitetsscreening system til at opdage hidtil ukendte forbindelser mod Mycobacterium tuberculosis, målretning intracellulære og i-bouillon voksende bakterier samt cytotoksicitet mod pattedyrværtscelle.

Tags

Infektion , Højkapacitetsscreening luciferase assay THP1 makrofag intracellulære MTT
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