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Identification of Plasmodesmal Localization Sequences in Proteins In Planta
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Immunology and Infection
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JoVE Journal Immunology and Infection
Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

DOI:
10.3791/55301-v

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08:07 min

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August 15, 2017

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, ,
1Department of Biochemistry and Cell Biology,State University of New York, 2Department of Plant Pathology and Plant-Microbe Biology,Cornell University

Chapters

  • 00:05Title
  • 00:59Plant Growth
  • 01:48Agroinfiltration
  • 04:46Confocal Microscopy
  • 06:03Results: Identification of Plasmodesmal Localization Sequences in Proteins
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

Plant intercellular connections, the plasmodesmata (Pd), play central roles in plant physiology and plant-virus interactions. Critical to Pd transport are sorting signals that direct proteins to Pd. However, our knowledge about these sequences is still in its infancy. We describe a strategy to identify Pd localization signals in Pd-targeted proteins.

Tags

Keywords: PlasmodesmataLocalization SignalIntercellular TransportProtein TargetingNicotiana BenthamianaFluorescent TaggingAgrobacterium TumefaciensTransient Expression

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