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Immunology and Infection

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JoVE Journal Immunology and Infection
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
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Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

Article DOI: 10.3791/55319-v • 08:32 min • March 10th, 2017
March 10th, 2017 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1
1Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, Department of Medicine, University of Miami Leonard M. Miller School of Medicine, 2Department of Cell Biology and Anatomy, University of Miami Leonard M. Miller School of Medicine
* These authors contributed equally

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Summary
Automatic Translation

Here, we show an enzymatic approach to isolate primary hepatocytes from adult mice, and we describe the quantification of an inflammatory response using ELISA and real-time PCR.

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Inflammatory Response Primary Hepatocyte Cultures Mice Isolation Functional Murine Hepatocytes Expression Secretion Inflammatory Cytokines CRP IL6 Hepatic Acute Phase Response Hepatocellular Function Chronic Kidney Disease Technique Robust Tool Analysis Hepatic Metabolomics Inflammation Drugs Liver Perfusion Medium Liver Digest Medium Water Bath Perfusion Pump Tubing System Air Bubbles Stereotactic Microscope Anesthetizing Absorbent Pad Adhesive Tape Extremities Fixation Shave Disinfect Abdomen Surgical Scissors Ventral Laparotomy Abdominal Wall Incision
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