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Biochemistry

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Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
 

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

Article doi: 10.3791/55349
March 26th, 2017

Summary March 26th, 2017

This protocol describes the reconstitution of nucleosomes containing differentially isotope-labeled sister histones. At the same time, asymmetrically post-translationally modified nucleosomes can be generated after using a premodified histone copy. These preparations can be readily used to study modification crosstalk mechanisms, simultaneously on both sister histones, by using high-resolution NMR spectroscopy.

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