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Journal / Cancer Research / A Protocol for Rapid Post-mortem Cell Culture of…
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Cancer Research

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JoVE Journal Cancer Research
A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)
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A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)

Article DOI: 10.3791/55360-v • 08:46 min • March 7th, 2017
March 7th, 2017 •
1, 2
1Graduate Program in Neuroscience, Department of Neurology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, 2Departments of Neurology, Neurosurgery, Pathology and Pediatrics, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine

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Summary
Automatic Translation

This protocol describes a method for the rapid processing of post-mortem diffuse intrinsic pontine glioma samples for the establishment of patient-derived cell culture models or direct characterization of tumor and microenvironmental cells.

Tags

Post-mortem Cell Culture Diffuse Intrinsic Pontine Glioma DIPG Patient-derived Cell Cultures Therapeutic Strategies Genetic Subtypes Pediatric High-grade Gliomas Adult Glioblastomas Central Nervous System Tumors Sample Preparation Sterilization UV Light Sterile Conditions
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