Patient-derived Cell Culture
Patient-derived Cell Culture - Related Videos
12:16
Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
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Cited by 22 •
2013
This article describes the preparation of freshly obtained melanoma tissue into primary cell cultures, and how to remove contaminations of erythrocytes and fibroblasts from the tumor cells. Finally, we describe how CD133+ putative melanoma stem cells are sorted from the CD133- bulk using Magnetic Activated Cell Sorting (MACS).
09:24
Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing
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Cited by 20 •
2016
Patient-derived xenografts of glioblastoma multiforme can be miniaturized into living microtumors using 3D human biogel culture system. This in vivo-like 3D tumor assay is suitable for drug response testing and molecular profiling, including kinomic analysis.
06:48
In Ovo Xenografting of Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells (PDX-ALL)
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2025
This protocol describes in ovo xenografting of patient-derived B- and T-acute lymphoblastic leukemia (ALL) cells, which occurs 4 days following injection.
11:26
Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling
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Cited by 19 •
2015
This protocol describes techniques for live cell isolation and primary culture of myogenic and fibroblast cell lines from muscle or skin tissue. A technique for the immortalization of these cell lines is also described. Altogether, these protocols provide a reliable tool to generate and preserve patient-derived cells for downstream applications.
08:29
Establishment and Culture of Patient-Derived Breast Organoids
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Cited by 7 •
2023
A detailed protocol is provided here for establishing human breast organoids from patient-derived breast tumor resections or normal breast tissue. The protocol provides comprehensive step-by-step instructions for culturing, freezing, and thawing human patient-derived breast organoids.
FAQs
Patient-derived cell culture can preserve differences among individuals because the cells originate from distinct tumor or tissue samples. Those differences may appear in tumor behavior, cellular heterogeneity, disease mechanisms, and responses to anticancer drugs. Comparing cultures from multiple patients therefore helps investigators distinguish effects that are broadly reproducible from responses associated with an individual’s disease biology.
Cell survival and proliferation depend on maintaining controlled culture conditions. Nutrient-containing media supplies the immediate growth environment, while suitable environmental conditions support continued viability and expansion after tissue processing. If these requirements are not maintained, the culture may not provide enough cells for downstream cancer studies, making consistent handling central to obtaining interpretable results.
An important analytical value is the ability to examine cellular heterogeneity rather than treating a tumor as biologically uniform. A patient sample can contain cells with different characteristics, and culturing those cells allows researchers to investigate how that diversity relates to tumor behavior or drug response. This makes the model useful for connecting variation within a sample to cancer mechanisms.
The workflow begins with obtaining a patient tissue sample, followed by isolating and dissociating the cells. Investigators then seed the resulting cells in nutrient-containing culture media and maintain them under suitable environmental conditions. Once survival and proliferation are supported, the culture can be used to study tumor behavior, disease mechanisms, heterogeneity, or treatment response.
Investigators test anticancer drugs on cultures established from different individuals and compare the resulting therapeutic effects. Because each culture reflects the biology of its source tissue, differences in response can be examined across patients rather than inferred from a single model. These comparisons generate evidence about treatment variability and may inform research aimed at more personalized cancer therapy.
It is especially useful when researchers need a tissue-based system for studying how tumors behave, how disease mechanisms operate, and why anticancer treatments affect patients differently. The same culture framework can support investigations of cellular heterogeneity and drug response, linking laboratory observations to individual disease biology. It also contributes to developing therapies intended to be more effective across patient groups.