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A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)

A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)

DOI:
10.3791/55360-v

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08:46 min

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March 07, 2017

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1Graduate Program in Neuroscience, Department of Neurology, Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 2Departments of Neurology, Neurosurgery, Pathology and Pediatrics, Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine

Chapters

  • 00:05Title
  • 01:12Mechanical Dissociation
  • 03:25Enzymatic Dissociation
  • 04:50Sucrose Gradient Centrifugation
  • 05:49ACK Red Blood Cell Lysis and Initial Culture Maintenance
  • 06:50Results: Images of Primary Cultures and Early-passage Cells
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method for the rapid processing of post-mortem diffuse intrinsic pontine glioma samples for the establishment of patient-derived cell culture models or direct characterization of tumor and microenvironmental cells.

Tags

Keywords: Diffuse Intrinsic Pontine Glioma (DIPG)Post-mortem Cell CulturePatient-derived Cell CultureRapid ProcessingPediatric High-grade GliomasAdult GlioblastomasCentral Nervous System TumorsCell CultureSterile Technique

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