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A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
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Chapters
- 00:05Title
- 01:54Isolate C. difficile from Different Environmental or Clinical Sources
- 03:46Culturing C. difficile and Killing Kinetic Procedures
- 04:53Prepare Samples for Scanning Electron Microscopy
- 06:26Imaging C. difficile Cells on a Scanning Electron Microscope
- 09:21Image Processing and Analysis
- 10:08Results: Morphological Analysis of C. difficile in Response to Antibiotic Treatment
- 11:40Conclusion
Antibiotic efficacy is most commonly determined by conducting killing kinetic studies and measuring colony forming units (CFUs). By integrating scanning electron microscopy (SEM) with these standard methods, we can distinguish the pharmacological effects of treatment between different antibiotics.
