Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates

The overall goal of this procedure is to improve the scalability and reproducibility of uniform spheroid formation using a liquid overlay technique in 384 well plates. The main advantage of this technique is that it reduces excessive medium re-operation from multiple plates, and thereby improving the reproducibility of spheroid formation. We have developed and validated the method of culturing 3D spheroids for arthropod screening tests in our laboratory and our study was to evaluate what is the edge effect in the plates of the multicellular cultures.

Today I will demonstrate the procedure together with Sona, a poster from the laboratory. Begin this procedure by weighting out zero point seven five grams of low melting point agarose and add it to 100 milliliters of mccoy's 5a medium with or without phenol red and without serum. Heat the solution in a microwave and swirl it every one to two minutes to completely dissolve the agarose.