Journal
/
Cancer Research
/
Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates

Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates

DOI:
10.3791/55403-v

•

11:24 min

•

March 07, 2017

•

, , , ,
1Institute of Molecular and Translational Medicine,Palacky University in Olomouc

Chapters

  • 00:05Title
  • 00:58Preparation of Agarose-coated Plates
  • 03:06Cell Culture and Multicellular Tumor Spheroid Formation
  • 06:17Medium Exchange Using a Robotic System
  • 07:14High-content Imaging of MCTS and Semi-automated Image Analysis
  • 08:04Analysis of Multicellular Spheroid Formation in the Presence of Anti-cancer Drugs
  • 10:16Conclusion

Summary

Automatic Translation

Automatic Translation

The uneven loss of medium from microtiter plates affects the reproducibility of uniform multicellular tumor spheroid formation. Improving culture conditions to reduce significant medium loss will improve the reproducibility of spheroid formation and the results of spheroid-based assays using the liquid-overlay technique.

Tags

Keywords: 3D Spheroid FormationMulticellular SpheroidsLiquid Overlay Technique384-well PlatesAgarose CoatingEvaporation ReductionReproducibilityScalabilityArthropod ScreeningEdge Effect

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55403?article_type=v

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures
check_url/55403?article_type=v

A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
check_url/55403?article_type=v

Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks
check_url/55403?article_type=v

Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma
check_url/55403?article_type=v

A Comprehensive Procedure to Evaluate the In Vitro Performance of the Putative Hemangioblastoma Neovascularization Using the Spheroid Sprouting Assay
check_url/55403?article_type=v

A 3D Organotypic Melanoma Spheroid Skin Model
check_url/55403?article_type=v

Potentiation of Anticancer Antibody Efficacy by Antineoplastic Drugs: Detection of Antibody-drug Synergism Using the Combination Index Equation
check_url/55403?article_type=v

Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models
check_url/55403?article_type=v

A Spheroid Killing Assay by CAR T Cells
check_url/55403?article_type=v

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

Read Article