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Journal
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Bioengineering
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细胞外基质衍生的泡沫和微载体如组织特异性细胞培养和交付平台的制造
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms
Please note that all translations are automatically generated.
Click here for the English version.
细胞外基质衍生的泡沫和微载体如组织特异性细胞培养和交付平台的制造
DOI:
10.3791/55436-v
•
11:19 min
•
April 11, 2017
•
Anna Kornmuller
,
Cody F.C. Brown
,
Claire Yu
,
Lauren E. Flynn
4
1
Biomedical Engineering Graduate Program
,
The University of Western Ontario
,
2
Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry
,
The University of Western Ontario
,
3
Department of Chemical Engineering
,
Queen’s University
,
4
Department of Chemical & Biochemical Engineering, Faculty of Engineering
,
The University of Western Ontario
Chapters
00:05
Title
00:52
Decellularized Tissue Processing
01:41
Extracellular Matrix (ECM) Suspension Preparation
04:01
ECM-derived Foam Fabrication
04:58
ECM-derived Microcarrier Fabrication via Electrospraying
07:11
Preparation of Foams and Microcarriers for Cell Culture
08:55
Results: Fabricated ECM-derived Foams and Microcarriers
10:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
组织特异性细胞外基质(ECM)是细胞功能的关键介质。本文介绍了合成纯ECM衍生的泡沫,并且在培养中稳定,而不需要化学交联
在体外
细胞培养模型在先进的3D应用程序或为促再生的生物支架的微载体的方法。
Tags
Extracellular Matrix
ECM
Foams
Microcarriers
3D Cell Culture
Decellularization
Cryomilling
Alpha Amylase
NaH2PO4 Buffer
NaCl Wash
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