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A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry
JoVE Journal
Biology
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JoVE Journal Biology
A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry
DOI:

05:57 min

March 28, 2017

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Chapters

  • 00:05Title
  • 00:43Staining
  • 02:16Microscopy
  • 03:45Muscle Fiber Quantification
  • 04:43Results: Representative Muscle Fiber Analyses of a Rat Muscle Cross Section
  • 05:27Conclusion

Summary

Automatic Translation

Here, we present a protocol for rapid muscle fiber analyses, which allows improved staining quality, and thereby automatic acquisition and quantification of fiber populations using the freely available software ImageJ.

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