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A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry
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A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry

A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry

DOI:
10.3791/55441-v

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05:57 min

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March 28, 2017

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, , , , , ,
1CD Laboratory for the Restoration of Extremity Function, Division of Plastic and Reconstructive Surgery, Department of Surgery,Medical University of Vienna, 2Core Facility Imaging, Core Facilities,Medical University Vienna, 3Department of Hand, Plastic and Reconstructive Surgery, Burn Center, BG Trauma Center Ludwigshafen, Plastic and Hand Surgery,University of Heidelberg

Chapters

  • 00:05Title
  • 00:43Staining
  • 02:16Microscopy
  • 03:45Muscle Fiber Quantification
  • 04:43Results: Representative Muscle Fiber Analyses of a Rat Muscle Cross Section
  • 05:27Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for rapid muscle fiber analyses, which allows improved staining quality, and thereby automatic acquisition and quantification of fiber populations using the freely available software ImageJ.

Tags

Muscle Fiber AnalysisImmunohistochemistryMyosin Heavy ChainRat MuscleAutomated ProtocolImage AcquisitionDAPINeuromuscularAgingDiseaseTraumaSkeletal MuscleCross-sectionQuantificationOpen-sourceSlide ScannerImage Processing

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