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JoVE Journal
Biochemistry

This content is Open Access.

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
 

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Article DOI: 10.3791/55498
April 1st, 2017

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Summary April 1st, 2017

Here we present protocols for affinity purification of protein complexes and their separation by blue native PAGE, followed by protein correlation profiling using label free quantitative mass spectrometry. This method is useful to resolve interactomes into distinct protein complexes.

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