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Immunology and Infection

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An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses

Article DOI: 10.3791/55499-v • 08:16 min • July 7th, 2017
July 7th, 2017 •
*1, *2, *3, 3, 4, 5,2, 6, 7, 2, 8, 1
1Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory "D. Lissandrini", University of Verona, 2Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., 3Department of Diagnostic and Public Health, Microbiology Division, University of Verona, 4Dipartimento di Scienze Medico-Veterinarie, University of Parma, 5Department of Biomedical Biotechnological and Translational Sciences, University of Parma, 6Department of Computer Science, University of Verona, 7Department of Neurological, Biomedical and Movement Sciences, University of Verona, 8Cystic Fibrosis Regional Center (CFC), AOUI Verona
* These authors contributed equally

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Summary
Automatic Translation

The method described here allows for the visualization of IL-8 promoter-dependent inflammation activation in the lungs of mice through non-invasive bioluminescence imaging (BLI). The same animal can be subjected to BLI multiple times for up to two months from the time of delivery of the luciferase reporter construct.

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IL-8 Transiently Transgenized Mouse Model In Vivo Monitoring Inflammatory Responses Lung Inflammation Noninvasive Challenge Pro-inflammatory Stimulus Bacterial Culture Supernatant Host-pathogen Interactions Respiratory Infection Diseases Advantages Of The Technique Visual Demonstration Gene Delivery Nucleic Acid Complex Tail Warming Tail Vein Injection Restraining Device Injection Site Pressure Intraperitoneal Injection D-Luciferin
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