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Biochemistry

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JoVE Journal Biochemistry
Protein Kinaz Aktivitesini Radyoaktif Etiketli ATP ile Tahlil
A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
Journal (Biochemistry)
A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
Journal (Biochemistry)
Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
 
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Protein Kinaz Aktivitesini Radyoaktif Etiketli ATP ile Tahlil

Article DOI: 10.3791/55504
May 26th, 2017 •
1, 1, 1
1Department of Pharmacology, University of Texas Southwestern Medical Center

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May 26th, 2017

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Protein kinazlar, inter-ve intraselüler sinyal iletimi için kritik olan, oldukça gelişen sinyalleme enzimleri ve iskeletleridir. Radyoaktif işaretli adenosin trifosfatın ([γ- 32 P] ATP) kinaz aktivitesini ölçmek için bir protokol sunuyoruz, bu hücresel sinyal düzenlenmesinin aydınlatılmasına yardımcı olan güvenilir bir yöntemdir.

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