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Journal / Developmental Biology / A Simple Method to Identify Kinases That Regulate …
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Developmental Biology

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JoVE Journal Developmental Biology
A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
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A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Article DOI: 10.3791/55515-v • 07:18 min • May 12th, 2017
May 12th, 2017 •
1, 2,3, 1
1The MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, 2Department of Cancer Biology, Dana-Farber Cancer Institute, 3Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School

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Summary
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Here, we present a quantitative and scalable protocol to perform targeted small molecule screens for kinase regulators of the naïve-primed pluripotent transition.

Tags

Kinases Embryonic Stem Cells Pluripotency High-throughput Inhibitor Screening Protein Kinase Inhibitors Signaling Pathways Technique Laboratory Equipment Reagents Gelatin-coated Dishes Mouse ES Cell Line LIF Fetal Calf Serum Knockout Serum Replacement Culture Cells Passage
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