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A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

DOI:
10.3791/55515-v

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07:18 min

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May 12, 2017

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, ,
1The MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences,University of Dundee, 2Department of Cancer Biology,Dana-Farber Cancer Institute, 3Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School

Chapters

  • 00:05Title
  • 00:47mESC Culture Conditions and Procedures for Screen Preparation
  • 03:09Kinase Inhibitor Screening Analysis
  • 04:56Results: Identification of Kinase Inhibitors that Modulate mESC Pluripotency
  • 06:13Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a quantitative and scalable protocol to perform targeted small molecule screens for kinase regulators of the naïve-primed pluripotent transition.

Tags

Keywords: Embryonic Stem CellPluripotencyKinase Inhibitor ScreeningHigh-throughputNovel Signaling PathwaysMouse ES CellsGelatin CoatingCell CultureTrypsinizationCell Counting96-well PlateKinase Inhibitor Treatment

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