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CAPRRESI: Chimären Assembly durch Plasmid Recovery und Restriktion Enzym Site Insertion

Article DOI: 10.3791/55526-v • 07:37 min • June 25th, 2017
June 25th, 2017 •
1, 2, 3
1Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, 2Oncomedic Incorporation, Ciudad de México, 3Laboratorio Internacional de Investigación sobre el Genoma Humano, Universidad Nacional Autónoma de México

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Hier präsentieren wir die Chimären-Assemblierung durch Plasmid-Wiedergewinnung und Restriktionsenzym-Insertion (CAPRRESI), ein Protokoll, das auf der Insertion von Restriktionsenzymstellen in Synonym-DNA-Sequenzen und funktionelle Plasmid-Recovery basiert. Dieses Protokoll ist eine schnelle und kostengünstige Methode zum Fixieren von Protein-kodierenden Genen.

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Molekulare Biologie Ausgabe 124 Chimäre Protein Kodierung Gen Assembly Synonym DNA Sequenz Insertion Restriktion Enzym Website Insertion Plasmid Recovery PUC18 / 19 Vektor Antibiotika Resistenz Gen Disruption Gentechnik Fusion Protein primäre Sigma Faktor RpoD SigA
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