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A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
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Biochemistry
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JoVE Journal Biochemistry
A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

DOI:
10.3791/55558-v

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08:03 min

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March 28, 2017

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, , ,
1Department of Biology,Georgetown University, 2Division of Infectious Diseases,Weill Cornell Medical College, 3Department of Medicine,Weill Cornell Medical College

Chapters

  • 00:05Title
  • 00:50Establishment of Steady-state S. aureus Growth
  • 02:39Sample Collection Setup and Sample Harvest
  • 04:54Metabolite Extraction
  • 05:54Results: Comparison of Wild Type S. aureus Cells to an Isogenic Mutant Lacking the Global Transcriptional Regulator, CodY
  • 07:20Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a protocol for the extraction of metabolites from Staphylococcus aureus and their subsequent analysis via liquid chromatography and mass spectrometry.

Tags

Keywords: Tandem Liquid Chromatography-Mass SpectrometryMetabolite AnalysisStaphylococcus AureusMetabolismPathogenesisNutrient DepletionHost InfectionOptical DensityExtraction BufferQuenching Solution

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