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JoVE Journal
Immunology and Infection

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An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
 

An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

Article DOI: 10.3791/55559
May 8th, 2017

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Summary May 8th, 2017

Here we describe a rapid equilibrium dialysis (RED) method to measure drug binding to caseum from pulmonary tuberculosis lesions and cavities. The protocol is also used with a foamy macrophage-derived matrix that is an effective surrogate to caseum.

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