Lcms Analysis
Lcms Analysis - Related Videos
09:38
Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method
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Cited by 88 •
2013
Knowledge of the composition of the phloem sap as well as the mechanism of its loading and long-distance transport is essential for the understanding of long-distance signaling in plant development and stress/pathogen response and of assimilate transport. This manuscript describes the collection of phloem exudates using the EDTA-facilitated method.
06:04
LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
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Cited by 7 •
2016
Here we show isolated human platelets can be used as an accessible ex vivo model to study metabolic adaptations in response to the complex I inhibitor rotenone. This approach employs isotopic tracing and relative quantification by liquid chromatography-mass spectrometry and can be applied to a variety of study designs.
09:26
Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
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Cited by 6 •
2011
This protocol will demonstrate the extraction and analysis of free and esterified bioactive fatty acids from cells. Fatty acids are accurately quantified using stable isotope dilution, chiral liquid chromatography, electron capture atmospheric chemical ionization multiple reaction monitoring mass spectrometry (SID-LC-ECAPCI-MRM/MS).
07:21
Automated Sample Preparation for the Multiplexed Analysis of Single-Cell Histone Post-Translational Modifications (sc-hPTM2)
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Cited by 1 •
2025
Here, we present a protocol to automate single-cell histone post-translational modification analysis using an isotopic two-plex labeling strategy and ArgC digestion. This workflow enables quantitative, reproducible, and high-sensitivity profiling of chromatin heterogeneity and epigenetic responses at single-cell resolution.
15:04
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
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Cited by 46 •
2009
This article describes a novel protocol and reagent set designed for sensitive measurement of neurotoxic effects of compounds and treatments on co-cultures of neurons and astrocytes using high content analysis. Results demonstrate that high content analysis represents an exciting novel technology for neurotoxicity assessment.
FAQs
Separation in the liquid chromatography stage depends on how each compound interacts with the stationary phase and the solvent. Compounds therefore pass through the system at different points, allowing the instrument to resolve components in a complex biological mixture before mass analysis. This separation improves the ability to associate detected signals with particular sample constituents.
Mass spectrometry adds molecular specificity after chromatographic separation. The separated molecules are ionized, and the instrument measures their mass-to-charge ratios, producing information that supports compound detection, identification, measurement, and structural characterization. Using both stages is valuable because retention behavior and mass-related information provide complementary evidence when biological samples contain many compounds.
LCMS analysis can examine metabolites, lipids, peptides, proteins, and drug compounds because its two stages provide different kinds of information. Liquid chromatography resolves the mixture, while mass spectrometry evaluates the resulting ions by mass-to-charge ratio. This combination supports examination of chemically diverse molecules within biological samples rather than focusing on only one compound class.
A biological sample such as blood, tissue, or a cell extract is introduced for chromatographic separation. The separated components then undergo ionization, and their mass-to-charge ratios are measured. The resulting measurements can be used to detect, identify, and measure compounds and to support structural characterization, depending on the biological question.
Biologists use LCMS analysis when they need to examine molecular changes across pathways, identify potential biomarkers, investigate disease mechanisms, or assess responses to treatment. Its application to blood, tissue, and cell extracts connects chemical measurements with biological states. The method can therefore support studies that relate molecular composition to disease processes or therapeutic effects.
LCMS results can provide evidence about which compounds are present, how much of a compound is present, and what structural characteristics may distinguish it. In biological research, these outcomes help organize information about metabolites, lipids, peptides, proteins, or drugs and connect measured molecules with pathways, biomarkers, disease mechanisms, or treatment responses.