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JoVE Journal
Developmental Biology

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A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
 

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

Article DOI: 10.3791/55565 10:07 min May 17th, 2017
May 17th, 2017

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Summary

We present a method to quantify DNA methylation based on the 5-methylcytosine (5-mC) dot blot. We determined the 5-mC levels during chondrocyte dedifferentiation. This simple technique could be used to quickly determine the chondrocyte phenotype in ACI treatment.

Transcript

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5-mC Dot Blot Assay DNA Methylation Level Chondrocyte Dedifferentiation Cell-based Cartilage Defect Treatment Autologous Chondrocyte Implantation Reliable Method Rapid Method General DNA Methylation Level Chondrocyte Phenotype Technology Implication Cartilage Defects Osteoarthritis Dot Blot Method Demonstration Method Genomic DNA Denaturation Serial Dilution Positively-charged Nylon Membrane Zhaofeng Jia Articular Cartilage Tissues
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