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Novel Assay for Cold Nociception in Drosophila Larvae
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Novel Assay for Cold Nociception in Drosophila Larvae
DOI:

06:52 min

April 03, 2017

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Chapters

  • 00:05Title
  • 00:56Preparation of Larvae
  • 02:31Cold Probe Assay
  • 04:36Results: Larvae Exhibit Unique Behaviors in Response to a Noxious Cold Stimulus
  • 05:36Conclusion

Summary

Automatic Translation

Here we demonstrate a novel assay to study cold nociception in Drosophila larvae. This assay utilizes a custom-built Peltier probe capable of applying a focal noxious cold stimulus and results in quantifiable cold-specific behaviors. This technique will allow further cellular and molecular dissection of cold nociception.

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