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Analisi delle sospensioni cellulari isolate da tessuti solidi da Spectral citometria a flusso
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Analisi delle sospensioni cellulari isolate da tessuti solidi da Spectral citometria a flusso

Article DOI: 10.3791/55578-v • 11:08 min • May 5th, 2017
May 5th, 2017 •
1, 2,3,4,5, 2, 1
1Flow Cytometry Core Facility, Center for Translational Research-Technical Core, Institut Pasteur, 2Unit for Lymphopoiesis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur, 3Stem-Cell Microenvironments in Repair/Regeneration Team, Instituto de Investigação e Inovação em Saúde (i3s), INEB - Instituto de Engenharia Biomédica, 4ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 5Stem Cells and Regenerative Medicine Team, UMRS 1166, ICAN - Institute of Cardiometabolism And Nutrition, UPMC - Université Pierre et Marie Curie - Paris 6, INSERM

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Questo articolo descrive spettrale citometria, un nuovo approccio per citometria di flusso che utilizza le forme di spettri di emissione per distinguere fluorocromi. Un algoritmo sostituisce compensazioni e può trattare auto-fluorescenza come parametro indipendente. Questo nuovo approccio permette la corretta analisi delle cellule isolate da organi solidi.

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Biologia Cellulare Numero 123 citometria a flusso spettrale auto-fluorescenza intestino cuore embrionale analisi multi-parametrica
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