A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Flow Cytometry Core Facility, Center for Translational Research-Technical Core,Institut Pasteur, 2Unit for Lymphopoiesis, Immunology Department, INSERM U1223,University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur, 3Stem-Cell Microenvironments in Repair/Regeneration Team,Instituto de Investigação e Inovação em Saúde (i3s), INEB – Instituto de Engenharia Biomédica, 4ICBAS – Instituto de Ciências Biomédicas Abel Salazar,Universidade do Porto, 5Stem Cells and Regenerative Medicine Team, UMRS 1166, ICAN – Institute of Cardiometabolism And Nutrition,UPMC – Université Pierre et Marie Curie – Paris 6, INSERM
Chapters
- 00:05Title
- 01:07Intestinal Cells Isolation
- 02:20Cell Staining
- 03:26Preparation of Cell Suspension from Embryonic Mouse Heart
- 05:31Cardiac Cell Staining
- 06:54Data Acquisition and Analysis
- 08:44Results: Spectral Cytometry-based Characterization of Embryonic Cardiac Cells
- 09:50Conclusion
This article describes spectral cytometry, a new approach in flow cytometry that uses the shapes of emission spectra to distinguish fluorochromes. An algorithm replaces compensations and can treat auto-fluorescence as an independent parameter. This new approach allows for the proper analysis of cells isolated from solid organs.
