Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

The overall goal of this procedure is to induce targeted ablation of Zebrafish kidney epithelia cells to model nephron injury and repair. This method can help answer key questions in the organ and specifically kidney regeneration field. Such as, what are the major cellular processes involved in regeneration, what is the extent of plasticity of surviving tissues and many more.

The main advantage of this technique is that it allows for precise spatial temporal control of injury, as well as the ability to adjust the level of injury. The implications of this technique extend toward therapy or diagnosis of acute kidney injury, because it allows precise spatial and temporal assessment of cellular and molecular processes involved in kidney cell injury and regeneration. To begin, generate embryos by crossing kidney GFP fluorescent Zebrafish.