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Journal
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Biochemistry
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通过共沉淀测量蛋白与F-肌动蛋白的结合
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Measuring Protein Binding to F-actin by Co-sedimentation
Please note that all translations are automatically generated.
Click here for the English version.
通过共沉淀测量蛋白与F-肌动蛋白的结合
DOI:
10.3791/55613-v
•
06:17 min
•
May 18, 2017
•
Jonathon A. Heier
,
Daniel J. Dickinson
,
Adam V. Kwiatkowski
1
Department of Cell Biology
,
University of Pittsburgh School of Medicine
,
2
Department of Biology
,
University of North Carolina at Chapel Hill
Chapters
00:05
Title
00:33
Prepare the Test Protein and F-actin for the Assay
02:02
Pelleting Assay – Quantification
04:33
Results: αE-catenin Homodimer Binds F-actin
05:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方案描述了一种测试蛋白质与丝状肌动蛋白(F-肌动蛋白)共沉淀的能力的方法,并且如果观察到结合,则测量相互作用的亲和力。
Tags
Protein Binding
F-actin
Co-sedimentation
Actin Polymerization
Ultracentrifugation
Protein Concentration
Binding Assay
Affinity Measurement
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