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Journal / Biochemistry / Medición de la unión de proteínas a actina F por…
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Biochemistry

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JoVE Journal Biochemistry
Medición de la unión de proteínas a actina F por co-sedimentación
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Medición de la unión de proteínas a actina F por co-sedimentación

Article DOI: 10.3791/55613-v • 06:17 min • May 18th, 2017
May 18th, 2017 •
1, 2, 1
1Department of Cell Biology, University of Pittsburgh School of Medicine, 2Department of Biology, University of North Carolina at Chapel Hill

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Este protocolo describe un método para probar la capacidad de una proteína para co-sedimentar con actina filamentosa (F-actina) y, si se observa unión, para medir la afinidad de la interacción.

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Bioquímica Edición 123 actina actina filamentosa F-actina proteína de unión a actina co-sedimentación ensayo de granulación curva de unión
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