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Developmental Biology
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二维和三维胚体小鼠胚胎干细胞的维甲酸诱导神经分化的分析
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies
Please note that all translations are automatically generated.
Click here for the English version.
二维和三维胚体小鼠胚胎干细胞的维甲酸诱导神经分化的分析
DOI:
10.3791/55621-v
•
09:04 min
•
April 22, 2017
•
Junning Yang
,
Chuanshen Wu
,
Ioana Stefanescu
,
Arie Horowitz
3
1
Department of Medicine, Cardeza Vascular Research Center
,
Sidney Kimmel Medical College, Thomas Jefferson University
,
2
Department of Molecular Cardiology
,
Cleveland Clinic Foundation
,
3
Department of Cancer Biology, Cardeza Vascular Research Center
,
Sidney Kimmel Medical College, Thomas Jefferson University
Chapters
00:05
Title
00:51
Embryoid Body Formation
01:58
2D Embryoid Body Culture and Detection of Secreted Proteins
03:04
3D Embryoid Body Culture
04:06
Embryoid Body Dissociation
05:56
Analysis of Embryoid Body Formation by Immunofluorescence
07:12
Results: Characteristics of Embryo Body Cultures and Visualization of Neural Differentiation Markers
08:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了使用小鼠胚胎干细胞生成二维或三维的胚状体的技术。然后,我们解释如何以诱导胚状体细胞视黄酸,以及如何的神经分化由祖细胞标志物的免疫荧光和免疫印迹来分析其分化状态。
Tags
Embryonic Stem Cells
Neural Differentiation
Retinoic Acid
Embryoid Bodies
Gene Expression
Protein Markers
Optical Microscopy
Trypsin-EDTA
IMDM
FBS
Hanging Drops
Fibronectin-coated Coverslips
Protein Secretion Analysis
Centrifugal Filters
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