/
/
Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:47Embryo Mounting and Insertion into Sample Chamber
- 04:48Light Sheet-based Fluorescence Microscopy
- 06:08Embryo Retrieval Quality Control and Image Data Processing
- 08:05Results: Representative Embryo Imaging Analyses
- 09:42Conclusion
Imaging the morphogenesis of insect embryos with light sheet-based fluorescence microscopy has become state of the art. This protocol outlines and compares three mounting techniques appropriate for Tribolium castaneum embryos, introduces two novel custom-made transgenic lines well suited for live imaging, discusses essential quality controls and indicates current experimental limitations.
