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Cancer Research
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利用DNA染色体引发的侧群体表型,从生物样品中检测和纯化癌细胞
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Cancer Research
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JoVE Journal
Cancer Research
Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples
Please note that all translations are automatically generated.
Click here for the English version.
利用DNA染色体引发的侧群体表型,从生物样品中检测和纯化癌细胞
DOI:
10.3791/55634-v
•
09:57 min
•
May 10, 2017
•
Maximilian Boesch*
1,2,3
,
Elisabeth Hoflehner
3
,
Dominik Wolf
3,4
,
Guenther Gastl
,
Sieghart Sopper*
2,3
1
Institute of Immunobiology
,
Kantonsspital St. Gallen
,
2
Internal Medicine V
,
Medical University of Innsbruck
,
3
Tyrolean Cancer Research Institute (TKFI)
,
4
Medical Clinic III, Oncology, Hematology, Immunoncology and Rheumatology
,
University Clinic Bonn (UKB)
Chapters
00:05
Title
01:41
Cell Preparation
02:51
Sample Preparation and Staining
05:44
Flow Cytometry Analysis
07:24
Results: Identification and Characterization of Side Population Cells
08:20
Conclusion
Summary
Automatic Translation
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Automatic Translation
允许从生物样品中鉴定和分离干细胞群体的方法对于癌症及其以外的干细胞靶向治疗的进展至关重要。在这里,我们提供使用染料触发侧群体表型的癌症干细胞分离的详细方案。
Tags
DNA Dye
Side Population
Cancer Stem Cells
ABC Drug Transporters
Cell Culture
Cell Suspension
Trypsin-EDTA
Hemocytometer
Violet Dye
Inhibition Controls
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