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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
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Biochemistry
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JoVE Journal Biochemistry
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

DOI:
10.3791/55655-v

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09:29 min

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May 18, 2017

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, , , ,
1Department of Cell Biology and Physiology, School of Medicine,University of North Carolina at Chapel Hill

Chapters

  • 00:05Title
  • 00:49Preparation of Detergent-soluble and High-salt Extracts of Tissue-specific IFs
  • 03:16Automated Tissue Lysis for RNA and IF Protein Extraction in High-volume Experiments
  • 07:15Results: High-salt Extraction of Intermediate Filament Proteins Using Lysing Matrix
  • 08:50Conclusion

Summary

Automatic Translation

Automatic Translation

In this method, we present biochemical procedures for rapid and efficient isolation of intermediate filament (IF) proteins from multiple mouse tissues. Isolated IFs can be used to study changes in post-translational modifications by mass spectrometry and other biochemical assays.

Tags

Keywords: Intermediate Filament ProteinsPost-translational ModificationsTriton X-100 BufferHigh Salt BufferPBS EDTA BufferSDS Sample BufferCentrifugationHomogenization

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