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Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
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Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

DOI:
10.3791/55656-v

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09:33 min

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May 12, 2017

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, , , , ,
1Division of Rheumatology, Immunology, and Allergy,Brigham and Women’s Hospital, 2Department of Medicine,Harvard Medical School, 3Department of Histology and Embryology,Poznan University of Medical Sciences, 4The Broad Institute of Harvard and M.I.T.

Chapters

  • 00:05Title
  • 00:52Stressing Cells
  • 02:19Cell Fixation and Staining
  • 05:23Fluorescence In Situ Hybridization
  • 07:43Results: Stress Granules Formation in Sodium Arsenite- and Vinorelbine-treated Cells
  • 08:46Conclusion

Summary

Automatic Translation

Automatic Translation

Stress Granules (SGs) are nonmembranous cytoplasmic structures that form in cells exposed to a variety of stresses. SGs contain mRNAs, RNA-binding proteins, small ribosomal subunits, translation-related factors, and various cell signaling proteins. This protocol describes a workflow that uses several experimental approaches to detect, characterize, and quantify bona fide SGs.

Tags

Keywords: Cytoplasmic FociStress GranulesMammalian CellsU-2 OS CellsSodium ArseniteVinorelbineCycloheximidePuromycinImmunofluorescenceCell FixationCell Permeabilization

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