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Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
 

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Article doi: 10.3791/55656
May 12th, 2017

Summary May 12th, 2017

Stress Granules (SGs) are nonmembranous cytoplasmic structures that form in cells exposed to a variety of stresses. SGs contain mRNAs, RNA-binding proteins, small ribosomal subunits, translation-related factors, and various cell signaling proteins. This protocol describes a workflow that uses several experimental approaches to detect, characterize, and quantify bona fide SGs.

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