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Flash-and-Freeze
Western Blotting: Sample Preparation to Detection
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Western Blotting: Sample Preparation to Detection
Thermal Limits Determination for Zooplankton Using a Heat Block
Journal (Biology)
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Flash-and-Freeze: En ny teknik för att fånga Membrane Dynamics med elektronmikroskopi

Article DOI: 10.3791/55664-v • 10:01 min • May 1st, 2017
May 1st, 2017 •
*1,2, *1, 1,3
1Department of Cell Biology, Johns Hopkins School of Medicine, 2Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, 3Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
* These authors contributed equally

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Vi utvecklade en ny teknik i elektronmikroskopi, "flash-och frys" som möjliggör visualisering av membrandynamiken med ms tidsupplösning. Denna teknik kombinerar optogenetic stimulering av nervceller med högtrycks frysning. Här visar vi de förfaranden och beskriva protokollen i detalj.

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Cellulär Biology elektronmikroskopi optogenetik synapse membrandynamik fryssubstitution channelrhodopsin högtrycks frysning exocytos endocytos tidsupplöst elektronmikroskopi flash-och-frys
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