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Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups
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Biochemistry
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JoVE Journal Biochemistry
Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups

Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups

DOI:
10.3791/55677-v

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06:44 min

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April 06, 2017

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, ,
1Department of Chemistry,Slippery Rock University

Chapters

  • 00:05Title
  • 00:57Hydrolysis of Ni-Schiff-Base Complex
  • 02:05Fmoc Protection of Hydrolyzed Amino Acid
  • 03:57Results
  • 05:32Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present an efficient hydrolysis and subsequent Fmoc protection of an amino acid isolated from a Ni-Schiff-base complex. Hydrolysis conditions presented here are suitable for use when retention of acid-labile side-chain protecting groups is required. This technique may be adaptable to a variety of unnatural amino acid substrates.

Tags

Keywords: Ni-Schiff-Base ComplexHydrolysisAcid-labile Protecting GroupsUnnatural Amino AcidsFmoc ProtectionEDTASolvent ExtractionPH Adjustment

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