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Cancer Research
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Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy

Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy

DOI:
10.3791/55709-v

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08:28 min

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December 27, 2017

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, , , , , ,
1Institut Cochin, Inserm U1016-CNRS UMR8104,Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals,Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center,University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals,University Paris Descartes

Chapters

  • 00:05Title
  • 01:01Agarose Embedding and Vibratome Slicing of Human Lung Tumors
  • 03:51Immunostaining of Resident CD8 T Cells and Tumor Cells
  • 05:07Imaging and Analyzing Resident CD8 T Cell Migration Within Human Lung Tumor Slices
  • 07:03Results: Distribution and Motility Patterns of Resident CD8 T Cells in Human Lung Tumor Tissues
  • 07:53Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method to image resident tumor-infiltrating CD8 T cells labeled with fluorescently coupled antibodies within human lung tumor slices. This technique permits real-time analyses of CD8 T cell migration using confocal microscopy.

Tags

Keywords: Ex Vivo ImagingResident CD8 T LymphocytesHuman Lung Tumor SlicesConfocal MicroscopyTumor Infiltrating CD8 T CellsTumor ImmunologyT Cell MigrationAgaroseVibratome

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