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Immunology and Infection
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用于维持和区分生物型的实验室技术<em>霍乱弧菌</em>临床和环境隔离
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Laboratory Techniques Used to Maintain and Differentiate Biotypes of
Vibrio cholerae
Clinical and Environmental Isolates
Please note that all translations are automatically generated.
Click here for the English version.
用于维持和区分生物型的实验室技术<em>霍乱弧菌</em>临床和环境隔离
DOI:
10.3791/55760-v
•
07:58 min
•
May 30, 2017
•
Kyle D. Brumfield*
1
,
Bailey M. Carignan*
1
,
Jordan N. Ray
,
Panagiota E. Jumpre
,
Mike S. Son
1
Department of Biological Sciences
,
Plymouth State University
Chapters
00:05
Title
01:28
Preparation and Use of Frozen Stock Cultures
02:46
Growth of Overnight Cultures in Liquid Medium
03:23
Phenotypic Assays for Biotype Classification via Spotting
04:04
Motility Assay
04:48
Voges-Proskauer (VP) Assay
05:47
Results: Reliable Distinction Between Biotypes
07:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该手稿除了进行一系列生物化学测定之外,还描述了适用于
霍乱弧菌的
维护技术,它们共同用于临床和环境
V
之间的快速可靠的分化。
霍乱弧菌
生物型实验室设置。
Tags
Vibrio Cholerae
Clinical Isolates
Environmental Isolates
Biotyping
Genotypic Characterization
Phenotypic Characterization
Biochemical Assays
Metabolic Capabilities
Enteric Bacteria
Laboratory Techniques
Culture Maintenance
Cryogenic Storage
Agar Plating
Liquid Culture
Inoculation
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