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Developmental Biology
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中等规模的制备<em>果蝇</em>蛋白质组学实验胚胎提取物
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Developmental Biology
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JoVE Journal
Developmental Biology
Medium-scale Preparation of
Drosophila
Embryo Extracts for Proteomic Experiments
Please note that all translations are automatically generated.
Click here for the English version.
中等规模的制备<em>果蝇</em>蛋白质组学实验胚胎提取物
DOI:
10.3791/55804-v
•
09:16 min
•
May 30, 2017
•
Liu Yang
,
Sayantanee Paul
,
Sarah DuBois-Coyne
,
Phillip Kyriakakis
,
Alexey Veraksa
1
Biology Department
,
University of Massachusetts Boston
,
2
Department of Bioengineering
,
University of California, San Diego
Chapters
00:05
Title
01:06
Preparation of 5 Liter and 1 Liter Fly Cages and Collection of Fly Embryos
04:12
Embryo Dechorionation
05:15
Preparation of Embryo Extract
07:15
Results: Preparation of
Drosophila
Embryo Extracts for Proteomics Experiments
08:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方案的目标是提供一种直接和廉价的方法来收集中等规模的
果蝇
胚胎(0.5-1 g),并制备可用于下游蛋白质组学应用的蛋白质提取物,如亲和纯化 - 质谱(AP-MS )。
Tags
Drosophila
Embryo
Proteomics
Protein Extraction
Affinity Purification
Mass Spectrometry
Cell Signaling
Developmental Biology
Medium-scale
User-friendly
Fly Cage
Apple Juice Plates
Yeast Paste
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